Yuav ua li cas thiaj tsis lees paub cov protein: 7 Kauj Ruam (nrog Duab)

Cov txheej txheem:

Yuav ua li cas thiaj tsis lees paub cov protein: 7 Kauj Ruam (nrog Duab)
Yuav ua li cas thiaj tsis lees paub cov protein: 7 Kauj Ruam (nrog Duab)

Video: Yuav ua li cas thiaj tsis lees paub cov protein: 7 Kauj Ruam (nrog Duab)

Video: Yuav ua li cas thiaj tsis lees paub cov protein: 7 Kauj Ruam (nrog Duab)
Video: yuav coj li cas kom luag nyiam 2024, Lub peb hlis ntuj
Anonim

Tej zaum koj xav kom tsis lees paub cov protein rau txoj haujlwm tshawb fawb, lossis tej zaum koj tau nyeem txog cov zaub mov tsis raug thiab xav paub tias ua haujlwm li cas. Denaturation yog txheej txheem uas cov protein poob nws cov duab thiab cov qauv vim los ntawm kev ua los ntawm sab nrauv, suav nrog cua sov, hluav taws xob, kua qaub, thiab cov kuab tshuaj. Cov kuab tshuaj organic feem ntau tsis muaj cov protein. Qhov tseeb uas koj tsis lees paub cov protein nyob ntawm qhov tshwj xeeb uas koj xav ua haujlwm nrog, vim tias lawv txhua tus xav tau cov neeg sawv cev sib txawv ntawm qib sib txawv. Feem ntau ntawm cov protein tuaj yeem ua tsis tau, txawm li cas los xij, yog tias koj paub hloov dab tsi hauv cov protein ib puag ncig.

Cov kauj ruam

Txoj Kev 1 ntawm 2: Tshaj tawm cov protein

Denature Protein Kauj Ruam 1
Denature Protein Kauj Ruam 1

Kauj Ruam 1. Siv cua sov

Tshav kub yog ib txoj hauv kev yooj yim tshaj plaws thiab feem ntau txoj hauv kev rau denature protein. Thaum cov protein nyob hauv nqe lus nug muaj nyob hauv cov zaub mov, tsuas yog ua zaub mov noj yuav ua rau cov protein tsis zoo. Ntau cov protein tuaj yeem ua rau tsis txawv txav los ntawm kev nthuav tawm lawv mus rau qhov kub siab lossis siab dua 100 ° C (212 ° F). Qhov no tso cai rau cov protein kom coagulate thiab txo lawv cov solubility.

Qhov ntev ntawm kev raug nyob ntawm qhov koj tab tom nthuav tawm kom sov. Piv txwv li, ib lub qe tuaj yeem ua noj hauv lub lauj kaub ntawm cov cua sov nruab nrab hauv tsib feeb, thaum ci tuaj yeem siv sijhawm teev ua noj hauv qhov cub

Denature Protein Kauj Ruam 2
Denature Protein Kauj Ruam 2

Kauj Ruam 2. Thov cawv

Dej cawv cuam tshuam rau cov nyiaj hydrogen uas tshwm sim ntawm amide pawg ntawm peptide bond. Thaum cov protein nthuav tawm rau kev daws cawv, cov cawv cawv tsim cov ntawv tshiab nrog cov protein txuas. Siv cov tshuaj 70% cawv los tsoo los ntawm cov phab ntsa ntawm cov kab mob thiab tsis lees paub cov protein.

Cov cawv uas muaj cawv ntau tuaj yeem ua rau muaj kev phom sij, vim tias lawv tuaj yeem hlawv thiab muaj tshuaj lom. Ib txwm hnav tag nrho cov khoom siv nyab xeeb suav nrog hnab looj tes thiab tiv thaiv qhov muag, thiab tuav hauv qhov chaw nyab xeeb, tswj qhov kub thiab txias

Denature Protein Kauj Ruam 3
Denature Protein Kauj Ruam 3

Kauj Ruam 3. Hloov pH

Cov txheej txheem sab hauv ntawm cov protein tuaj yeem tawg thaum nws ib puag ncig muaj acidic lossis alkaline heev, vim tias lawv cuam tshuam cov ionic sib txuas uas tuav cov protein ntsev ntsev txuas ua ke. Ntxiv cov kua qaub los yog kua tov rau cov protein. Ib puag ncig ib puag ncig yuav tsum nyob ntawm pH siab dua 10 lossis qis dua 4 txhawm rau txhawb kev tsis lees paub. Yog tias koj yaum kom hloov pauv nrog cov kua qaub, pH yuav tsum nyob nruab nrab ntawm 2 txog 5.

Hloov pauv pH tuaj yeem ua kom muaj cov amino acid, nyob ntawm pKa ntawm txhua pab pawg ua haujlwm hauv cov amino acid ntawd. Koj tseem tuaj yeem ionize cov pab pawg amino lossis pawg carboxyl tam sim no hauv cov amino acid

Denature Protein Kauj Ruam 4
Denature Protein Kauj Ruam 4

Kauj Ruam 4. Sim ntsev ntsev hlau hnyav

Cov hlau hnyav tuaj yeem cuam tshuam cov nyiaj hauv cov protein, ua rau nws poob nws cov qauv. Cov ntsev ntawm cov hlau hnyav xws li mercury thiab txhuas tuaj yeem siv los ua kom cov protein sib txawv. Cov ntsev zoo li no muaj los ntawm cov neeg siv tshuaj lom neeg feem ntau, thiab yuav tsum tau siv nrog kev ceev faj thiab cov cuab yeej nyab xeeb kom raug suav nrog hnab looj tes thiab tiv thaiv qhov muag.

Cov hlau hnyav tuaj yeem cuam tshuam nrog cov protein muaj nuj nqi sab ua haujlwm pab pawg los tsim cov complexes. Cov hlau hnyav tseem oxidize cov protein cov amino acid sab saw

Txoj Kev 2 ntawm 2: Hloov kho cov protein

Denature Protein Kauj Ruam 5
Denature Protein Kauj Ruam 5

Kauj Ruam 1. Txiav txim seb cov protein puas tuaj yeem rov qab tau

Qee cov qauv ntawm denaturation yog mus tas li, thaum lwm tus tuaj yeem ua tsis tau. Piv txwv li ua noj qe lossis nqaij, piv txwv li, tsis tuaj yeem thim rov qab, tab sis cov protein uas tau nthuav tawm rau pH siab tuaj yeem rov zoo li qub thaum muab tso rau hauv ib puag ncig zoo dua.

Txawm hais tias cov protein tuaj yeem rov qab ua tau yuav nyob ntawm nws cov DNA. DNA yuav tuav cov ntaub ntawv xav tau rau cov protein kom rov qab mus rau nws lub xeev qub

Denature Protein Kauj Ruam 6
Denature Protein Kauj Ruam 6

Kauj Ruam 2. Tshem tawm qhov tsis txaus ntseeg

Rov qab ib puag ncig ib puag ncig cov protein rau stasis thiab tshem tawm cov ntsiab lus tseem ceeb. Piv txwv, tshem cov kua qaub los yog hauv paus, lossis nqa cov protein rov qab mus rau qhov ntsuas kub kom tsim nyog.

Denature Protein Kauj Ruam 7
Denature Protein Kauj Ruam 7

Kauj Ruam 3. Siv cov khoom siv renaturation

Ntau lub tuam txhab cov khoom siv kuaj sim muag cov khoom siv renaturation uas tso cai rau koj tshuaj xyuas qhov ntsuas tsis zoo tshaj plaws los txhawb kev rov ua dua tshiab. Cov khoom siv no tuaj yeem pab tau tshwj xeeb tshaj yog tias koj tab tom saib cov protein hauv chav kuaj lossis chaw teeb tsa.

Lub tswv yim

  • Cov protein uas tsis muaj peev xwm poob nws qhov kev nyob ib puag ncig, qib siab, thiab cov qauv theem ob uas pom tseeb hauv nws lub xeev ib txwm muaj, tab sis nws thawj cov qauv yuav nyob twj ywm.
  • Cov protein tsis zoo kuj poob qee qhov ntawm nws cov bioactivity, qee qhov, zoo li nrog cov enzymes, poob nws lub peev xwm los ua haujlwm. Piv txwv li, cov substrates yuav tsis tuaj yeem txuas tau rau cov enzymes uas tau ua tsis ncaj ncees lawm.
  • Cov protein uas tsis muaj peev xwm yuav plam nws qhov hnyav hauv kev daws teeb meem.
  • Koj tseem tuaj yeem tsis lees paub cov kua qaub nucleic siv cov txheej txheem kuaj sim cais cov kab.

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